dapi thermofisher d1306 (Thermo Fisher)
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dapi thermofisher d1306
Dapi Thermofisher D1306, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi+thermofisher+d1306/dapi/bio_rxiv__2025__07__16__665006-252-0-1
Average 90 stars, based on 1 article reviews
Dapi Thermofisher D1306, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi+thermofisher+d1306/dapi/bio_rxiv__2025__07__16__665006-252-0-1
Average 90 stars, based on 1 article reviews
dapi thermofisher d1306 - by Bioz Stars,
2026-10
90/100 stars
Images
1) Product Images from "Divergent Cell-Type Specific Hypoxia Responses in Human Stem Cell–Derived and Primary Islets"
Article Title: Divergent Cell-Type Specific Hypoxia Responses in Human Stem Cell–Derived and Primary Islets
Journal: bioRxiv
doi: 10.1101/2025.07.16.665006
Figure Legend Snippet: Divergent functional, metabolic, and cellular responses of primary and SC-islets to acute hypoxia. Functional, metabolic, and immunohistochemical analyses of primary islets (top row, A-E) and SC-islets (bottom row, F-J) after exposure to 1% O 2 for 0, 6, 24, or 48 hours. (A, F) Representative immunohistochemical images of (A) primary and (F) SC-islet sections at each timepoint. Panels show co-staining for DAPI (blue), C-peptide (Cpep, green), and PDX1 (red); DAPI, glucagon (GCG, green), and somatostatin (SST, cyan); and DAPI and BNIP3L (red). Scale bars = 200 µm. (B, G) Absolute insulin secretion in response to low (2 mM, grey bars) and high (20 mM, dark bars) glucose for (B) primary and (G) SC-islets. (C, H) Oxygen consumption rate (OCR) of (C) primary and (H) SC-islets, measured via Seahorse metabolic flux analysis. Different colored lines represent islets recovered after the indicated duration of hypoxia. The traces show metabolic responses to sequential injections (indicated by dashed lines) of glucose (20 mM), oligomycin (1.5 µM), FCCP (0.5 µM), and finally rotenone/antimycin A (0.5 µM). (D, I) Glucose-stimulated insulin secretion (GSIS) stimulation index (SI) for (D) primary and (I) SC-islets, calculated as the ratio of high-to-low glucose secretion. (E, J) Extracellular acidification rate (ECAR) of (E) primary and (J) SC-islets, measured concurrently with OCR. *Data in (B, D, G, I) are presented as mean ± SEM of n=3 (primary) or n=4 (SC-islets) biological replicates; individual data points are shown as open circles. Data in (B, E, G, J) are representative traces from a single experiment, with lines showing the mean ± SEM of technical replicates. For insulin secretion (B, G) and stimulation index (D, I), statistical significance was assessed using repeated measures ANOVA followed by pairwise t-tests with Bonferroni correction for post-hoc analysis. Significance is denoted as *p < 0.05, **p < 0.01.
Techniques Used: Functional Assay, Immunohistochemical staining, Staining
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To identify synaptic markers of BD and lithium response, we measured the effects of lithium on induced pluripotent stem cell-derived neurons from BD patients and controls. Article Title: Inhibition of acyl-CoA synthetase long-chain isozymes decreases multiple myeloma cell proliferation and causes mitochondrial dysfunction. Article Snippet: To characterize apoptosis, MM cells were collected, washed 3 times with Cell Staining Buffer (BioLegend, Cat. No. 420201, San Diego, CA, USA) and stained with APC-Annexin V (1 : 20, BioLegend Cat. no. 640920), Imaging:Article Title: Transplantation of decellularized porcine kidney grafts repopulated with primary human cells demonstrates filtration function in pigs. Article Snippet: Slides were stained with Article Title: Divergent Cell-Type Specific Hypoxia Responses in Human Stem Cell–Derived and Primary Islets Article Snippet: Article Title: Enzyme-mediated proximity labeling reveals the co-translational targeting of DLGAP5 mRNA to the centrosome during mitosis Article Snippet: Following washing with PBST three times, the cells were stained with Article Title: Hyaluronan Mediates Cold-Induced Adipose Tissue Beiging. Article Snippet: After three more washes in TBST, the specimens were incubated with 300 nM Article Title: Influence of Alzheimer’s disease related neuropathology on local microenvironment gene expression in the human inferior temporal cortex Article Snippet: Article Title: VSV ∆M51 drives CD8 + T cell-mediated tumour regression through infection of both cancer and non-cancer cells. Article Snippet: Endotoxin-free OVA (SigmaAldrich, A5503) was then diluted in PBS and added to wells of MuTuDCs to achieve concentrations indicated in the results. Article Title: Synaptic protein expression in bipolar disorder patient-derived neurons implicates PSD-95 as a marker of lithium response. Article Snippet: Bipolar disorder (BD) is a severe mental illness characterized by recurrent episodes of depression and mania.. Lithium is the gold standard pharmacotherapy for BD, but outcomes are variable, and the relevant therapeutic mechanisms underlying successful treatment response remain uncertain.. To identify synaptic markers of BD and lithium response, we measured the effects of lithium on induced pluripotent stem cell-derived neurons from BD patients and controls. Article Title: Inhibition of acyl-CoA synthetase long-chain isozymes decreases multiple myeloma cell proliferation and causes mitochondrial dysfunction. Article Snippet: To characterize apoptosis, MM cells were collected, washed 3 times with Cell Staining Buffer (BioLegend, Cat. No. 420201, San Diego, CA, USA) and stained with APC-Annexin V (1 : 20, BioLegend Cat. no. 640920), Binding Assay:Article Title: Transplantation of decellularized porcine kidney grafts repopulated with primary human cells demonstrates filtration function in pigs. Article Snippet: Slides were stained with Article Title: Divergent Cell-Type Specific Hypoxia Responses in Human Stem Cell–Derived and Primary Islets Article Snippet: Article Title: Enzyme-mediated proximity labeling reveals the co-translational targeting of DLGAP5 mRNA to the centrosome during mitosis Article Snippet: Following washing with PBST three times, the cells were stained with Article Title: Hyaluronan Mediates Cold-Induced Adipose Tissue Beiging. Article Snippet: After three more washes in TBST, the specimens were incubated with 300 nM Article Title: Influence of Alzheimer’s disease related neuropathology on local microenvironment gene expression in the human inferior temporal cortex Article Snippet: Article Title: VSV ∆M51 drives CD8 + T cell-mediated tumour regression through infection of both cancer and non-cancer cells. Article Snippet: Endotoxin-free OVA (SigmaAldrich, A5503) was then diluted in PBS and added to wells of MuTuDCs to achieve concentrations indicated in the results. Article Title: Synaptic protein expression in bipolar disorder patient-derived neurons implicates PSD-95 as a marker of lithium response. Article Snippet: Bipolar disorder (BD) is a severe mental illness characterized by recurrent episodes of depression and mania.. Lithium is the gold standard pharmacotherapy for BD, but outcomes are variable, and the relevant therapeutic mechanisms underlying successful treatment response remain uncertain.. To identify synaptic markers of BD and lithium response, we measured the effects of lithium on induced pluripotent stem cell-derived neurons from BD patients and controls. Article Title: Inhibition of acyl-CoA synthetase long-chain isozymes decreases multiple myeloma cell proliferation and causes mitochondrial dysfunction. Article Snippet: To characterize apoptosis, MM cells were collected, washed 3 times with Cell Staining Buffer (BioLegend, Cat. No. 420201, San Diego, CA, USA) and stained with APC-Annexin V (1 : 20, BioLegend Cat. no. 640920), Incubation:Article Title: Transplantation of decellularized porcine kidney grafts repopulated with primary human cells demonstrates filtration function in pigs. Article Snippet: Slides were stained with Article Title: Divergent Cell-Type Specific Hypoxia Responses in Human Stem Cell–Derived and Primary Islets Article Snippet: Article Title: Enzyme-mediated proximity labeling reveals the co-translational targeting of DLGAP5 mRNA to the centrosome during mitosis Article Snippet: Following washing with PBST three times, the cells were stained with Article Title: Hyaluronan Mediates Cold-Induced Adipose Tissue Beiging. Article Snippet: After three more washes in TBST, the specimens were incubated with 300 nM Article Title: Influence of Alzheimer’s disease related neuropathology on local microenvironment gene expression in the human inferior temporal cortex Article Snippet: Article Title: VSV ∆M51 drives CD8 + T cell-mediated tumour regression through infection of both cancer and non-cancer cells. Article Snippet: Endotoxin-free OVA (SigmaAldrich, A5503) was then diluted in PBS and added to wells of MuTuDCs to achieve concentrations indicated in the results. Article Title: Synaptic protein expression in bipolar disorder patient-derived neurons implicates PSD-95 as a marker of lithium response. Article Snippet: Bipolar disorder (BD) is a severe mental illness characterized by recurrent episodes of depression and mania.. Lithium is the gold standard pharmacotherapy for BD, but outcomes are variable, and the relevant therapeutic mechanisms underlying successful treatment response remain uncertain.. To identify synaptic markers of BD and lithium response, we measured the effects of lithium on induced pluripotent stem cell-derived neurons from BD patients and controls. Article Title: Inhibition of acyl-CoA synthetase long-chain isozymes decreases multiple myeloma cell proliferation and causes mitochondrial dysfunction. Article Snippet: To characterize apoptosis, MM cells were collected, washed 3 times with Cell Staining Buffer (BioLegend, Cat. No. 420201, San Diego, CA, USA) and stained with APC-Annexin V (1 : 20, BioLegend Cat. no. 640920), Microscopy:Article Title: Transplantation of decellularized porcine kidney grafts repopulated with primary human cells demonstrates filtration function in pigs. Article Snippet: Slides were stained with Article Title: Divergent Cell-Type Specific Hypoxia Responses in Human Stem Cell–Derived and Primary Islets Article Snippet: Article Title: Enzyme-mediated proximity labeling reveals the co-translational targeting of DLGAP5 mRNA to the centrosome during mitosis Article Snippet: Following washing with PBST three times, the cells were stained with Article Title: Hyaluronan Mediates Cold-Induced Adipose Tissue Beiging. Article Snippet: After three more washes in TBST, the specimens were incubated with 300 nM Article Title: Influence of Alzheimer’s disease related neuropathology on local microenvironment gene expression in the human inferior temporal cortex Article Snippet: Article Title: VSV ∆M51 drives CD8 + T cell-mediated tumour regression through infection of both cancer and non-cancer cells. Article Snippet: Endotoxin-free OVA (SigmaAldrich, A5503) was then diluted in PBS and added to wells of MuTuDCs to achieve concentrations indicated in the results. Article Title: Synaptic protein expression in bipolar disorder patient-derived neurons implicates PSD-95 as a marker of lithium response. Article Snippet: Bipolar disorder (BD) is a severe mental illness characterized by recurrent episodes of depression and mania.. Lithium is the gold standard pharmacotherapy for BD, but outcomes are variable, and the relevant therapeutic mechanisms underlying successful treatment response remain uncertain.. To identify synaptic markers of BD and lithium response, we measured the effects of lithium on induced pluripotent stem cell-derived neurons from BD patients and controls. Article Title: Inhibition of acyl-CoA synthetase long-chain isozymes decreases multiple myeloma cell proliferation and causes mitochondrial dysfunction. Article Snippet: To characterize apoptosis, MM cells were collected, washed 3 times with Cell Staining Buffer (BioLegend, Cat. No. 420201, San Diego, CA, USA) and stained with APC-Annexin V (1 : 20, BioLegend Cat. no. 640920), FACS:Article Title: Transplantation of decellularized porcine kidney grafts repopulated with primary human cells demonstrates filtration function in pigs. Article Snippet: Slides were stained with Article Title: Divergent Cell-Type Specific Hypoxia Responses in Human Stem Cell–Derived and Primary Islets Article Snippet: Article Title: Enzyme-mediated proximity labeling reveals the co-translational targeting of DLGAP5 mRNA to the centrosome during mitosis Article Snippet: Following washing with PBST three times, the cells were stained with Article Title: Hyaluronan Mediates Cold-Induced Adipose Tissue Beiging. Article Snippet: After three more washes in TBST, the specimens were incubated with 300 nM Article Title: Influence of Alzheimer’s disease related neuropathology on local microenvironment gene expression in the human inferior temporal cortex Article Snippet: Article Title: VSV ∆M51 drives CD8 + T cell-mediated tumour regression through infection of both cancer and non-cancer cells. Article Snippet: Endotoxin-free OVA (SigmaAldrich, A5503) was then diluted in PBS and added to wells of MuTuDCs to achieve concentrations indicated in the results. Article Title: Synaptic protein expression in bipolar disorder patient-derived neurons implicates PSD-95 as a marker of lithium response. Article Snippet: Bipolar disorder (BD) is a severe mental illness characterized by recurrent episodes of depression and mania.. Lithium is the gold standard pharmacotherapy for BD, but outcomes are variable, and the relevant therapeutic mechanisms underlying successful treatment response remain uncertain.. To identify synaptic markers of BD and lithium response, we measured the effects of lithium on induced pluripotent stem cell-derived neurons from BD patients and controls. Article Title: Inhibition of acyl-CoA synthetase long-chain isozymes decreases multiple myeloma cell proliferation and causes mitochondrial dysfunction. Article Snippet: To characterize apoptosis, MM cells were collected, washed 3 times with Cell Staining Buffer (BioLegend, Cat. No. 420201, San Diego, CA, USA) and stained with APC-Annexin V (1 : 20, BioLegend Cat. no. 640920), Flow Cytometry:Article Title: Transplantation of decellularized porcine kidney grafts repopulated with primary human cells demonstrates filtration function in pigs. Article Snippet: Slides were stained with Article Title: Divergent Cell-Type Specific Hypoxia Responses in Human Stem Cell–Derived and Primary Islets Article Snippet: Article Title: Enzyme-mediated proximity labeling reveals the co-translational targeting of DLGAP5 mRNA to the centrosome during mitosis Article Snippet: Following washing with PBST three times, the cells were stained with Article Title: Hyaluronan Mediates Cold-Induced Adipose Tissue Beiging. Article Snippet: After three more washes in TBST, the specimens were incubated with 300 nM Article Title: Influence of Alzheimer’s disease related neuropathology on local microenvironment gene expression in the human inferior temporal cortex Article Snippet: Article Title: VSV ∆M51 drives CD8 + T cell-mediated tumour regression through infection of both cancer and non-cancer cells. Article Snippet: Endotoxin-free OVA (SigmaAldrich, A5503) was then diluted in PBS and added to wells of MuTuDCs to achieve concentrations indicated in the results. Article Title: Synaptic protein expression in bipolar disorder patient-derived neurons implicates PSD-95 as a marker of lithium response. Article Snippet: Bipolar disorder (BD) is a severe mental illness characterized by recurrent episodes of depression and mania.. Lithium is the gold standard pharmacotherapy for BD, but outcomes are variable, and the relevant therapeutic mechanisms underlying successful treatment response remain uncertain.. To identify synaptic markers of BD and lithium response, we measured the effects of lithium on induced pluripotent stem cell-derived neurons from BD patients and controls. Article Title: Inhibition of acyl-CoA synthetase long-chain isozymes decreases multiple myeloma cell proliferation and causes mitochondrial dysfunction. Article Snippet: To characterize apoptosis, MM cells were collected, washed 3 times with Cell Staining Buffer (BioLegend, Cat. No. 420201, San Diego, CA, USA) and stained with APC-Annexin V (1 : 20, BioLegend Cat. no. 640920), |
